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STEMCELL Technologies Inc cd8 depletion kit easysep

Cd8 Depletion Kit Easysep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd8+depletion+kit+easysep/cd8+depletion+kit+easysep/pmc06497464-256-15-19
Average 90 stars, based on 1 article reviews
cd8 depletion kit easysep - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Antigen receptor control of methionine metabolism in T cells"

Article Title: Antigen receptor control of methionine metabolism in T cells

Journal: eLife

doi: 10.7554/eLife.44210


Figure Legend Snippet:

Techniques Used: Activation Assay, Staining, Blocking Assay, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Methylation, Adjuvant, Incubation, Software

Related Articles

Activation Assay:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Staining:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Blocking Assay:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Recombinant:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Isolation:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Enzyme-linked Immunosorbent Assay:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Methylation:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Adjuvant:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Incubation:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Software:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Fluorescence:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Concentration Assay:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Flow Cytometry:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Expressing:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg

Infection:

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cytokines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg/ml)

Article Title: Antigen receptor control of methionine metabolism in T cells
Article Snippet: ThermoFisher Scientific, UK) in the presence of cyto- kines IL12 (10 ng/ml; RnD Systems, UK) and 20 ng/ml IL2 (20 ng/ml; Proleukin, Novartis, UK). .. To generate Th1s, murine CD8+ T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK). .. The resulting mix of CD4+ T cells and APC were cultured at 3 105 cells/ml for 5 days in the presence of anti-CD3 (2 mg/ml) and anti-CD28 (3 mg/ml) and

Article Title: Single cell analysis of kynurenine and System L amino acid transport in T cells
Article Snippet: generate CTLs, cells were washed from TCR stimulus after 48 h and then expanded in IL-2 (20 ng/ml; Proleukin, Novartis) for a further 3–5 days. .. To generate TH1s and TH17s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies). .. The resulting mix of CD4 + T cells and APC were cultured at 3 × 10 5 cells/ml for 5 days in the presence of anti-CD3 (2 μg/ml) and anti-CD28 (3 μg



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Image Search Results


Journal: eLife

Article Title: Antigen receptor control of methionine metabolism in T cells

doi: 10.7554/eLife.44210

Figure Lengend Snippet:

Article Snippet: To generate Th1s, murine CD8 + T cells were depleted from lymph node preparations using CD8 depletion kit (EasySep, STEMCELL Technologies, UK).

Techniques: Activation Assay, Staining, Blocking Assay, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Methylation, Adjuvant, Incubation, Software